Bioanalytical Core
Lab - Services:
Cytokine Analysis Facility
Shipping, Storage, and Processing of a variety of
samples: Our facility provides bar-coded collection
vials for the appropriate tracking of samples, from
the collection site, through shipping, receiving,
and storage at our facility, and specific analysis
of inflammatory markers.
The types of samples processed include, but are not
limited to:
* Gingival crevicular fluid (GCF)
* Serum or plasma
* Whole blood
* Cell culture media
Analyses performed: Enzyme linked immuno-assays (ELISA)
are currently available for analyses of human fluid
samples (e.g. GCF, serum):
* Inflammatory mediators (Interleukins, PGE2, sCD14,
sICAM-1, TXB2, TNF)
* Acute-phase reactants (CRP), Glucose & Lipid
panels, and HbAlc
* Oxidative stress markers (tot-8isoPGF2alpha, 9alpha-11betaPGF2)
* Others (endothelin-1, big endothelin-1).
Our facility also performs various ELISAs (e.g.,
SAA, porcine insulin) for non-human samples (mice,
rabbits, dogs, swine). New capabilities include: complete
lipid panels using Cholestech TDX, hsCRP and plasma
protein assays using Dade Behring BN II automated
Nephelometer, and multiplex assays using Luminex/BioPlex.
See also http://www.dent.unc.edu/research/facilities/cytokine.htm
Charges
Please use this spreadsheet
to calculate the approximate cost of assays. Some
assays may be paid for by the GCRC. Email the spreadsheet
to david_barrow@dentistry.unc.edu.
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